| Actor | | Character | |
| Susan Abernethy | | Magdalen Dacre, Viscountess Montegu | |
| Susan Adams | | Mildred Cooke, Lady Burleigh | |
| Heather Amaral | | Catherine Carey, Mistress Carey MOH | |
| Perry Ash | | Sir Edmond Tylney, Master of the Revels | |
| Eric Atkinson | | Christopher Hatton, Sir Christopher Hatton | |
| Kelly Bailey | | Elizabeth Fitzgerald/Fiennes, Baroness Clinton | |
| Doug Bingham | | William Paulet, 1st Marquess of Winchester | |
| Lisa Bingham | | Catherine Howard/Berkley, Baroness Berkley | |
| Charlie Cannaday | | William Brooke, 10th Baron Cobham | |
| Jessica Cannaday | | Frances Newton/Brooke, Baroness Cobham | |
| Rowan Cannaday | | Margaret Brooke, Mistress Meg | |
| Kim Cardenes | | Dorothy Broadbelt, Mistress Dorothy Broadbelt | |
| Christiana Carro | | Catherine Knyvette, Mistress Knyvette MOH | |
| Terri Casey | | Lady Elizabeth Hardwick, Countess of Shrewsbury | |
| Samuel Chaing | | Sir James Croft, Comptroller | |
| Julia DelJudge | | Magaret Butler/North, Baroness North | |
| Shawn Donohugh | | Nicholas Bullingham, Bullingham | |
| Victoria Febres | | Queens Household, | |
| Michelle Fennema | | Lettice Knollys, Mistress Knollys MOH | |
| Christina Forst | | Margaret Audley/Howard, Duchess of Norfolk | |
| Brendon Goodyear | | Thomas Heneage, Sir Thomas | |
| Karl Hamilton | | Henry Sidney, Sir Henry Sidney | |
| Gail Hansen | | Joan FitzAlan, Baroness Lumley | |
| Larry Hansen | | Henry Berkley, Baron Berkley | |
| Bella Hendricks | | Philidelphia Carey, Mistress Carey MOH | |
| John Hertz | | Lord Thomas Howard, Viscount Bindon | |
| Matthew Karrel | | William Howard, 1st Baron Howard of Effingham | |
| Emily Kennedy | | Anne Herbert/Talbot, Baroness Talbot | |
| Gary Kephart | | Henry Carey, 2nd Baron Hunsdon | |
| Sara Kocher | | Isabella Markham/Harrington, Lady Markham | |
| Charles Laura | | Francis Talbot, Baron Talbot | |
| Alexsandra Lewicki | | Dorothy Stafford, Lady Stafford | |
| Alicia Lewis | | Anne Russell, Mistress Russell MOH | |
| Cori Lockridge | | Catherine Carey/Knollys, Lady Knollys | |
| Jimmy Mares | | Gregory Fiennes, Baron Dacre | |
| David Martin | | Nicholas Bacon, Sir Nicholas Bacon | |
| Jess Miller | | Mistress Blanche Parry, Mistress Parry | |
| Kenneth Miller | | Henry Killigrew, Sir Henry | |
| Melinda Miller | | Catherine Carey, Baroness Howard of Effingham | |
| G. Ann Mitschek | | Mistress Katherine Ashley, Mistress Ashley | |
| Tyronesha Moncrease | | Queens Household, | |
| David Neil | | Sir Thomas Parry, Queen's Coffierer | |
| Charles Oversby | | Master William Camden, Master Camden | |
| Lydia Oversby | | Mistress Elizabeth Camden, Mistress Camden | |
| Richard Pierce | | William Cecil, Baron Burleigh | |
| Joshual Pirro | | Thomas Knyvette, Master Knyvette | |
| Colleen Rude | | Elizabeth Stanley/Parker, Baroness Morley | |
| Dan Rutherford | | Henry Fitzlan, Earl of Arundel | |
| Rachelle Sanders | | Ann Sackville/Fiennes, Baroness Dacre of the South | |
| Malcolm Scott | | Doctor John Dee, Doctor Dee | |
| Alyson Smith | | Mistress Mary Sydney, Mistress Sydney | |
| Renee Spahn | | Mistress Helena Von Snakenborg, Mistress Snakenborg | |
| Ariel Stahel | | Frances Radcliffe, Mistress Radcliffe MOH | |
| Brenda Stewart | | Anne Stanley/Stourton, Baroness Stourton | |
| DeeAnna Stewart | | Katherine DeVere/Windsor, Baroness Windsor | |
| David Taylor | | Barnaby Fitzpatrick, Baron of Upper Ossory | |
| Laura Willis-Kephardt | | Anne Morgan/Carey, Baroness Hunsdon | |