| Actor | Charactor |
| Susan Abernethy |
Lady Ursula Pole, (Dowager) Baroness Stafford
|
| Susan Adams |
Mildred Cooke, Lady Burleigh
|
| Heather Amaral |
Essex Rich, Mistress Essex
|
| Perry Ash |
Robert Carey, Master Carey
|
| Kelly Bailey |
Mistress Margaret Butler, Mistress Margaret
|
| Howard Bein |
Master Richard Tarlton, Master Tarlton
|
| Melissa Bein |
Margaret Mitchell, Lady Sadleir
|
| Doug Bingham |
Sir Thomas Radcliffe, KG, 3rd Earl of Sussex, Lord Sussex
|
| Lisa Bingham |
Mistress Catherine Howard, Mistress Howard
|
| Logan Bingham |
Master Edward Howard, Master Edward
|
| Trevor Binham |
Master Robert Radcliffe, Master Robert
|
| Andy Bradshaw |
Johannes (Gambolputy) Anders, German Ambassador
|
| Beth Bradshaw |
Mistress Katarina Anders, Mistress Anders
|
| Gail Burkett |
Winifred Pole, Lady Barrington
|
| Charlie Cannaday |
Master John Hall, Master John
|
| Fiona Cannaday |
Mistress Fiona Hall, Mistress Fiona
|
| Jessica Cannaday |
Mistress Penelope Hall, Mistress Penelope
|
| Rowan Cannaday |
Mistress Rowan Hall, Mistress Rowan
|
| Kris Carty |
Anne Cooke, Lady Bacon
|
| Terri Casey |
Lady Elizabeth Hardwick, Countess of Shrewsbury
|
| Eric Cheung |
Master (Captain) James Croft, Captain Croft
|
| Ariston Collander |
George Clifford, Earl of Cumberland
|
| DeeAnna Collander |
Margaret Russell, Countess of Cumberland
|
| Julia DelJudge |
Lady Philadelphia Carey, Baroness Scrope
|
| Michelle Fennema |
Mistress Elizabeth Vernon, Mistress Vernon
|
| Christina Forst |
Elizabeth Russell, Countess of Bath
|
| Brendon Goodyear |
Master John Harrington, Master Harrington
|
| Victoria Hanlin |
Her Majesty, Elizabeth Tudor, Her Majesty
|
| Larry Hansen |
Sir William Browne, Sir William
|
| Gail Hansen-Amos |
Lady Mary Talbot, Baroness Talbot
|
| John Hertz |
Lord Thomas Howard, Viscount Bindon
|
| Jennifer Humbert |
Mistress Mary Fitton, Mistress Fitton
|
| Alexsandra Lewicki |
Mistress Elinor Whyting, Mistress Whyting
|
| Cori Lockridge |
Catherine Carey, Baroness Howard of Effingham
|
| Jimmy Mares |
Master John Davies, Master Davies
|
| David Martin |
Master Edward Coke, Master Coke
|
| Jess Miller |
Mistress Blanche Parry, Mistress Parry
|
| Meinda Miller |
Dorothy Deveraux, Lady Deveraux
|
| G. Ann Mitschek |
Mistress Katherine Ashley, Mistress Ashley
|
| David Neil |
John Neville, Baron Neville
|
| Charles Oversby |
Master William Camden, Master Camden
|
| Lydia Oversby |
Mistress Elizabeth Camden, Mistress Camden
|
| Richard Pierce |
William Cecil, Baron Burleigh
|
| Marcia Pina |
Magdalen Newport, Lady Herbert
|
| Andrea Plasencia |
Alice Goodfellow, Mistress Alice
|
| Colleen Rude |
Lucy Somerset, Baroness Neville
|
| Rachelle Sanders |
Mistress Elizabeth Brydges, Maid of Honor
|
| Malcolm Scott |
Doctor John Dee, Doctor Dee
|
| Samantha Sherlock |
Margaret Vernon, Lady Stanley
|
| Alyson Smith |
Mistress Mary Sydney, Mistress Sydney
|
| Renee Spahn |
Lady Frances Cavendish, Lady Pierrepont
|
| Brenda Stewart |
Lady Margery Grey, Baroness
|
| David Taylor |
Sir Barnaby Fitzpatrick, Sir Barnaby
|
| Laura Willis-Kephardt |
Lady Penelope Devereux, Baroness Rich
|
| David Wine |
Lord Henry Stanley, Earl of Derby
|
| Mary Wine |
Lady Margaret Clifford, Countess of Derby
|
| Nathan Wine |
Master William Stanley, Master Stanley
|
| Nicholas Wine |
Ferdinado Stanley, Lord Strange
|